At that time, the early signs have been dismissed as particles, though the retrospective evaluation shown shared traits with the problematic cells in tradition. Tracing the infection assisted the lab establish more laboratory strategies for screening and security of assay-important mobile lines.
No matter if you’re a newbie or an expert, take a look at our resources to progress your research and abilities in flow cytometry.
Mix acoustic focusing technological know-how with substantial-parameter Examination for versatile spectral unmixing and traditional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating by using a high-speed brightfield camera for simultaneous higher throughput flow cytometry and high resolution brightfield imaging.
*Pricing is indicative only and is particularly subject to vary. Be sure to Call your Agilent consultant with any thoughts. The information established forth herein isn't a proposal or perhaps a motivation, each of which might be matter to credit rating approval, conditions and terms, and availability with Agilent. Agilent Economical Methods is on the market in picked international locations.
Also, it really is illuminating to find out the quantity of doublets slip via standard singlet gates, and fantastic to confirm the standing of sudden double-constructive events in my Evaluation."
Optimizing gating method. Even strong handbook singlet gating is mistake-vulnerable and stays a subjective conclusion level in Just about all flow cytometry assays. Imaging can be utilized to substantiate and change gates to include only solitary cells of curiosity.
Reliable picture quality even at superior throughput Acoustic focusing plus a large-pace digital camera Mix to picture these T cells consistently at reduced or higher flow prices. Quickly change concentration and digital camera configurations to fulfill experimental requirements.
Early signs of this contamination had at first been dismissed as debris. Backgating to find new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also permits you to use morphological capabilities to discover intriguing subpopulations that will not be apparent from flow cytometry knowledge by yourself.
Users can look at event illustrations or photos in true-time to substantiate sample quality, improve protocols, and record the morphology of each and every mobile populace determined for Examination. Imaging capture also Rewards from acoustic focusing, which helps you to place the cells in order that a pointy, centered image is attained.
AI-pushed automated image analysis With all the Attune Cytometric Computer software interprets party functions into unique morphology parameters that could be coupled with conventional fluorescence and scatter parameters to reveal hugely reproducible populace improvements and one of a kind subsets.
We Beforehand demonstrated the power of imaging Auto-T/Ramos cell interactions. Let’s check out just the populace of best fascination, the double optimistic situations, to learn more. We can now use extended morphological parameters (below, circularity and skewness of depth) to even more take a look at the features of these populations and refine gating on these occasions, increasing info robustness.
Leverage the strength of State-of-the-art technologies and innovation to speed up your discoveries. Have confidence in within the Attune Flow Cytometer relatives to provide the reliability, pace, and accuracy that set your perform aside.
No clean/no lyse leukocyte Evaluation with imaging. Cells ended up obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Pro UX/UI permits customers to teach AI products for expanded apps. (A) The “Prepare Product” aspect supplies annotation instruments and serious-time model coaching precision.
At the guts of every Attune Flow Cytometer lies our revolutionary acoustic focusing technologies, which makes use of audio waves to meticulously align cells in the flow mobile. This ground breaking approach surpasses standard hydrodynamic procedures, causing more quickly processing speeds, Outstanding details quality, and increased sample throughput.
Since the mobile line had get more info Beforehand been analyzed on the Attune CytPix Flow Cytometer, the researchers went again to the images and were in the position to doc the microbial infection no less than 5 times before.
Agilent Worth Assure guarantees you a minimum of a decade of instrument use within the date you purchase. In any other case, We're going to credit history you Along with the residual worth of the process towards an upgraded model.
What appeared to be a homogeneous population is definitely more various—an insight that should be regarded as when interpreting success.